Biofabrication of human breast epithelial spheroids
2019
Drexel University, Philadelphia, USA
3D human cancer models are growing in popularity, as they recapitulate better than 2D cultures several characteristics of the in vivo microenvironment. However, biofabrication techniques are still time-consuming and depend on complex cellular interactions within the model components. Thus, preformed 3D spheroids using alginate-based bioinks could be useful to create models that overcome some of these limitations. Here, human breast epithelial cell lines were bioprinted individually or in pre-formed spheroids of different bioinks in monoculture or co-culture with human endothelial cells. The results showed that individually printed breast cells formed spheroids only in Matrigel-based bioinks. Nevertheless, pre-formed spheroids maintained their normal cellular features and were more resistant to paclitaxel when cultured without endothelial cells. Overall, the researchers propose a simplified biofabrication approach for 3D modelling of human breast cancer that can rapidly recapitulate tumor microenvironment characteristics for drug screening or mechanistic studies.
Bioprinting of 3D breast epithelial spheroids for human cancer models
Alisa Morss Clyne
Added on: 10-12-2021
[1] https://iopscience.iop.org/article/10.1088/1758-5090/aafc49[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
