3D multicellular microfluidic model of invasive ductal carcinoma in situ
2015
University of Wisconsin, Madison, USA
In recent years, there is growing knowledge about the transition from ductal carcinoma in situ breast cancer to metastatic cancers. However, the mechanisms underlying this process are poorly understood. Currently, available models are difficult to use and have severe limitations to recapitulate the main structure and functions of human ductal carcinoma in situ. Here, a microscale microfluidic model with human epithelial cells, human ductal carcinoma in situ cells and human mammary fibroblasts is developed to investigate the invasive transition of cancer cells. The results showed that this model recapitulates key structures and functions of ductal carcinoma in situ and allows to study cell-cell junctions and cell polarity. Moreover, the co-culture with human mammary fibroblasts induced invasive behaviour in ductal carcinoma in situ cells. In this study, the researchers propose a model that enables the formation of mammary ducts in a 3D environment and faithfully recapitulates the effects of ductal carcinoma in situ, opening the door to elucidate underlying mechanisms and the effects of microenvironmental factors of the invasive behaviour of ductal carcinoma in situ.
Microfluidic model of ductal carcinoma in situ with 3D, organotypic structure
Kyung E Sung, David J Beebe
Added on: 10-06-2021
[1] https://bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1007-5[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
