Microfluidic 3D model to study solid tumor treatment efficiency
December 2018
University of Wisconsin, Madison, USA
Solid tumors present several difficulties when designing treatment strategies, including immunotherapies. The mechanisms underlying the action of different therapies against solid tumors remain elusive to study because of the limitations of currently available in vitro models. Here, a new microfluidic model is developed with 3D human breast cancer spheroids embedded in a 3D extracellular matrix and flanked by lumens lined with human endothelial cells. In this model, the results showed that perfused antibodies could rapidly diffuse into the matrix but were unable of penetrating the spheroid. However, natural killer cells were able to target the tumor and penetrate to the innermost layer attacking tumor cells. When both approaches were combined, cytotoxicity in the periphery of the tumor spheroid was enhanced. In this study, the researchers develop a new model that shows to be a potential tool to investigate the efficiency of immunotherapy in solid tumors.
Evaluating natural killer cell cytotoxicity against solid tumors using a microfluidic model
Jose M Ayuso
Added on: 10-05-2021
[1] https://www.tandfonline.com/doi/full/10.1080/2162402X.2018.1553477[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
