Platform development to study bone metastasis
2016
The George Washington University, Washington DC, USA
Bone metastasis is one of the most common complications of late-stage breast cancer. Thus, there is a need to develop in vitro models that integrate breast cancer cells with bone matrix components to improve the understanding of the mechanisms underlying breast cancer bone invasion. Here, in vitro 3D printed hydrogel-based bone matrices are used to co-culture directly, in 2D or 3D formats, or indirectly human breast cancer cells and human fetal osteoblasts. The results showed that indirect co-culture inhibited osteoblast proliferation while increasing breast cancer cell growth and IL-8 secretion in both cell types. Moreover, the direct 3D culture setup allowed the formation of multi-cellular spheroids. In this study, the researchers develop different 3D printed bone matrices setups that can be used to investigate the mechanisms underlying bone metastasis in vitro through different approaches.
A 3D printed nano bone matrix for characterization of breast cancer cell and osteoblast interactions
Lijie Grace Zhang
Added on: 10-04-2021
[1] https://iopscience.iop.org/article/10.1088/0957-4484/27/31/315103[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
