3D multicellular tumor model with defined tumor microenvironment
2016
Biotec TU Dresden, Dresden, Germany
There is growing evidence that the interactions between tumor microenvironment and tumor cells play a key role in cancer progression. Some of the extracellular matrix proteins present in the extracellular space are implicated in cancer cell survival and invasive behaviour and tumor cells often drive extracellular matrix remodelling. However, there is a lack of experimental platforms that can reliably replicate this interplay in an in vitro 3D context. Here, a new 3D model with a motif-defined hydrogel-based extracellular matrix is developed and combined with the culture of human breast or prostate cancer cells alone or in combination with endothelial and mesenchymal stromal cells. The results showed that the most aggressive human cancer cell line exhibited an invasive morphology with enhanced cell growth and endothelial cell infiltration in hydrogels with collagen I and laminin-111 motifs. In this study, the researchers present a new multicellular 3D model with precisely defined biochemical cues that could be used to further dissect the mechanisms underlying extracellular matrix modulation of cancer progression and potentially help to find new therapeutic strategies that interfere with cancer cell invasion.
3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments
Anna V Taubenberger
Added on: 10-04-2021
[1] https://www.sciencedirect.com/science/article/abs/pii/S1742706116301015[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
