Optimal conditions to expand ex vivo patients T cells
2016
Hospital de la Santa Creu i Sant Pau, Barcelona, Spain
Adoptive T cell therapy (ACT) represents a highly promising strategy to treat cancer. While this approach has been mostly based on the use of terminally differentiated effector T cells, recent studies indicate that the use of less differentiated T cells with extensive replicative capacity has greater engraftment and antitumor effect, in particular, the recently described memory stem T cells (TSCM). Methods to generate memory stem T cells ex vivo rely on peptides and cytokines during the culture period yet too strong stimulation may induce differentiation of memory stem T cells to effector memory T cells. In the present study, the researchers purified naïve T cells from healthy donors which were then cultured ex vivo in the presence of different combinations of peptides and cytokines during different times. T cells phenotype was then assessed by multiparametric flow cytometry. The study shows which are the optimal conditions to increase the frequencies and expansion of memory stem T cells.
A short CD3/CD28 costimulation combined with IL-21 enhance the generation of human memory stem T cells for adoptive immunotherapy
J. Briones
Added on: 09-15-2021
[1] https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-016-0973-y[2] https://data.jrc.ec.europa.eu/dataset/352f7dfd-05cf-434b-a96a-7e270dc76573
