Generation of a high throughput 3D spheroid tumor model
2015
Institute for Tumor Biology and Experimental Therapy, Frankfurt, Germany
Natural killer (NK) cells rapidly recognize and destroy malignantly transformed cells. Due to their natural ability to lyse tumor cells without prior sensitization, NK cells hold promise for cancer immunotherapy although tumor immune escape strategies might compromise NK cell activity. In the present study, the researchers generated an in vitro, high throughput 3D tumor spheroid model from human cervical carcinoma cell lines to study NK cell infiltration and immunosurveillance. The model allows for long-term observation of cell proliferation, NK cell infiltration and NK cell cytotoxicity and antitumor efficacy. Further, the model allows for isolation of the cells that manage to infiltrate the tumor mimicking spheroid. The model can be used for donor selection of cytotoxic lymphocytes, status quo determination of antitumor immunoreactivity, and preconditioning of a cancer patient prior to allogeneic cellular immunotherapy and thus help to personalize treatment.
Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids
Joachim Koch
Added on: 09-15-2021
[1] https://bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1321-y[2] https://data.jrc.ec.europa.eu/dataset/352f7dfd-05cf-434b-a96a-7e270dc76573
