In vitro blockade of specific markers to restore NK cell function to fight pancreatic cancer
October 2014
The first Affiliated Hospital of Nanjing Medical University, Nanjing, China
Anti-tumor effect of NK cells decreases during pancreatic cancer progression. In the present study, the researchers aimed at understanding better the regulatory pathways by which NK cells facilitate tumor immune escape. NK cells were isolated from a healthy donor and co-cultured with normal human pancreatic cells and human pancreatic cancer cells. NK cell function was determined by a flow cytometric analysis and bioassay absorption of secreted cytokines. Further, the expression levels of different markers of NK cells functional stages were assessed. The results showed that NK cells function was significantly downregulated following exposure to pancreatic cancer cells compared to normal pancreatic cells, yet blockade of the dysfunctional markers using specific drugs restored NK function. The results should be exploited to develop immunotherapy.
Elevation of MMP-9 and IDO induced by pancreatic cancer cells mediates natural killer cell dysfunction
Yi Zhu, Yi Miao
Added on: 09-14-2021
[1] https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-738[2] https://data.jrc.ec.europa.eu/dataset/352f7dfd-05cf-434b-a96a-7e270dc76573
