Mechanisms of dopaminergic differentiation in vitro
2017
Xuzhou Medical University, Xuzhou, China
Parkinson's disease is a neurodegenerative disorder characterized by a massive loss of dopaminergic neurons. Several pharmacological strategies are used to alleviate the symptoms of the disease, but there is not yet an effective cure for the disease. Recently, cell replacement therapy is growing in popularity as a potential strategy for dopaminergic regeneration in Parkinson's disease. However, the mechanisms of differentiation of dopaminergic neurons are not well-known. Here, a human neuroblastoma cell line treated with glial cell-derived neurotrophic factor is used to elucidate key factors in the differentiation of dopaminergic neurons, with a special focus on glucose-6-phosphatase. Firstly, the researchers defined the conditions for glial cell-derived neurotrophic factor treatment for the differentiation process. Afterwards, it was confirmed that glucose-6-phosphatase expression is significantly upregulated throughout the differentiation and propose three microRNAs as key factors in this process. These results could be confirmed at a protein level. Overall, in this study, glucose-6-phosphatase is validated as an important factor in dopaminergic differentiation from a human neuroblastoma cell line, which might contribute to the investigation of dopaminergic cells production for cell-based therapies in Parkinson's disease.
Glucose-6-phosphatase-α participates in dopaminergic differentiation
Dian-Shuai Gao
Added on: 09-02-2021
[1] https://www.tandfonline.com/doi/full/10.1080/01616412.2017.1348681[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
