Transcriptomic profile of human induced dopaminergic neurons
2016
Montana State University, Bozeman, USA
Parkinson's disease is characterized by a massive loss of dopaminergic neurons. Recently, cell replacement therapy through the generation of induced dopaminergic neurons from human pluripotent stem cells has been proposed as a potential treatment. In this study, the transcriptional profiles of induced cells are compared against human primary dopaminergic neurons. After protocol optimization, it was possible to produce human induced dopaminergic neurons. In cells derived from Parkinson's patients, it was possible to distinguish a small subset of genes with altered expression. The transcriptomic analysis showed that the expression profiles of induced dopaminergic cells and primary midbrain neurons had differences, especially in genes related to neuronal maturation. Here, the researchers propose an improved protocol to generate dopaminergic neurons from human pluripotent cells that, when differentiated, can retain Parkinson's related features to be used in disease modelling. However, the protocols need to be further improved to generate fully functional induced dopaminergic neurons that closely resemble primary cells before using these cells in cell replacement therapy.
Transcriptional comparison of human induced and primary midbrain dopaminergic neurons
Renee A Reijo Pera
Added on: 08-31-2021
[1] https://www.nature.com/articles/srep20270[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
