Limitations of a human in vitro blood-brain barrier model
October 2016
Radboud University Nijmegen Medical Center, Nijmegen, Netherlands
The integrity of the blood-brain barrier is an essential feature of a healthy central nervous system. Consequently, several pathologies involve a defective blood-brain barrier. Decreased clearance of amyloid-beta at the blood-brain barrier has been observed in pathologies related to amyloidogenesis. In this study, a human brain endothelial cell line is evaluated as a model for amyloid-beta clearance along the blood-brain barrier. This model could only regulate the permeability of large molecules, allowing for substantially increased permeability of small molecules. Moreover, barrier formation for amyloid-beta was completely absent along the basolateral to apical transport. Furthermore, the development of tight junctions was incomplete. Overall, the investigated model showed important limitations in the study of clearance of amyloid-beta at the blood-brain barrier. The researchers bring new information about the limitations of this human model that should be further investigated before it is applied to study pathologies that involve amyloidogenesis.
Limitations of the hCMEC/D3 cell line as a model for Aβ clearance by the human blood-brain barrier
Marcel M Verbeek
Added on: 08-17-2021
[1] https://onlinelibrary.wiley.com/doi/10.1002/jnr.23964[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
