Bioreactor platform for neuronal culture
2016
National Research Council of Italy, Rende, Italy
One of the main problems of neuronal culture "in vitro" modelling has been the heterogeneous nutrient distribution. To overcome this problem, this study presents a membrane bioreactor that allows having a homogeneous and controlled microenvironment. Using a human neuroblastoma cell line, the researchers could produce a complex 3D functional active neuronal network, as is shown by the oxygen uptake and glucose consumption and the expression of synaptophysin. Furthermore, this model could be used to model amyloid beta-induced neurotoxicity: inducing cytotoxicity and oxidative stress in neuronal cells. This toxic effect was prevented using crocin. The researchers propose a solution to have a constant nutrient flow that makes it possible to obtain functional neuronal cultures with a homogeneous microenvironment what could potentially improve "in vitro" drug testing and development for neurodegenerative diseases.
Neuronal membrane bioreactor as a tool for testing crocin neuroprotective effect in Alzheimer’s disease
Loredana De Bartolo
Added on: 08-09-2021
[1] https://www.sciencedirect.com/science/article/abs/pii/S1385894716300018[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
