Fluorescence-based assay to study cell proliferation
October 2014
Kyoto University, Kyoto, Japan
An assay is established to have continuous monitoring of cell proliferation of human breast cancer cells through Kaede protein fluorescence. With this technique, the study finds that interleukin-6 has a positive effect on cell proliferation and that it can promote cell proliferation of other cancer cells through paracrine mechanisms. The results show that this method can open new opportunities to study cell proliferation mechanisms of cancer cells.
An optical labeling-based proliferation assay system reveals the paracrine effect of interleukin-6 in breast cancer
Junji Itou
Junji Itou et al. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2015 [1]
EURL ECVAM [2]
Added on: 08-01-2021
[1] https://www.sciencedirect.com/science/article/pii/S0167488914003553[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
