New tool to isolate circulating tumor cells
October 2015
University of Southern California, Los Angeles, USA
To be able to capture circulating tumor cells, a method is presented in this study, combining epithelial cell adhesion molecule immunomagnetic separation with a fluorescence-activated cell sorting. When applying this method to diverse cultured human breast cancer cell lines or to human peripheral blood samples, it was possible to efficiently recover cells from all intrinsic subtypes of breast cancer except for claudin-low cell lines. The transcriptomic profile of isolated cells from cell lines was highly correlated to the bulk cell line expression signature but not to the peripheral blood isolated cells. This method allows for isolation and high-quality RNA isolation of circulating tumor cells to perform a specific subtype analysis.
EpCAM based capture detects and recovers circulating tumor cells from all subtypes of breast cancer except claudin-low
Julie E Lang
Added on: 07-31-2021
[1] https://www.oncotarget.com/article/5977/[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
