Coupling of image analysis platform to breast cancer models
2017
Ewha Womans University, Seoul, South Korea(1)
Ewha Womans University School of Medicine, Seoul, South Korea(2)
Ewha Womans University School of Medicine, Seoul, South Korea(2)
Improvement of mammosphere assays is achieved by coupling in vitro models of human breast cancer cells to a medium-throughput imaging workflow and automated analysis. Using this method, it was possible to screen a library of several protein kinase inhibitors to further do an in-depth analysis of the mechanisms of the selected drugs in human breast cancer stem cells. Several signalling pathways are identified as targets to tackle several properties of cancer stem cells. This new automated platform has shown a great potential for drug screening of inhibitors of breast cancer stem cell activity.
Screening of breast cancer stem cell inhibitors using a protein kinase inhibitor library
Eok-Soo Oh(1), Duk-Hee Kang(2)
Added on: 07-22-2021
[1] https://cancerci.biomedcentral.com/articles/10.1186/s12935-017-0392-z[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
