3D organotypic cultures with automated image analysis for drug screening
2014
University of Turku, Turku, Finland
The study presents the validation of a high-content workflow based on coupling 3D culture models with automated image analysis. This platform is used to quantify actin-target treatment-induced morphological changes of several human prostate and breast cancer lines embedded in 3D extracellular matrix structures. Through this approach, it was possible to identify three drugs that attenuate invasive dynamics and study their signalling mechanisms, showing that this model can be a very powerful tool for large-scale drug screening/discovery and target validation.
Quantification of dynamic morphological drug responses in 3D organotypic cell cultures by automated image analysis
Matthias Nees
Added on: 07-20-2021
[1] https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096426[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
