Microvascular chips for the characterization of cardiovascular diseases
2018
Central China Normal University, Wuhan, China(1)
Wuhan Polytechnic University, Wuhan, China(2)
Wuhan Polytechnic University, Wuhan, China(2)
Endothelial cells seeded in hydrogel can form perfusable microvasculature networks by a vasculogenesis-like process. This approach can be used to develop advanced in vitro models where the interactions between functional vasculature (characterized by mature vessel walls and tissue parenchyma) is critical to mimic pathophysiological processes (intra- and extravasation processes involving leukocytes and cancer cells). Diffusion and effects of therapeutics can be tested in complex microenvironments. The device allows the delivery of nutrients, and oxygen, as well as flow-induced mechanical stimuli into the luminal space of the endothelium.
Fine particulate matter (FPM) for example has been thought to be associated with cardiovascular disease. FPMs were introduced into this physiologically human vessel-like microenvironment. The vascular toxicity was evaluated and the results demonstrated that intravascular accumulation of FPM could enhance ROS (reactive oxygen species) generation which may further cause endothelial dysfunction by oxidative stress. This is associated with endothelial inflammation and thrombosis. This simple and versatile platform can be used for a wide range of applications in vascular physiology studies of particulate matter in the context of cardiovascular disease.
Functional human 3D microvascular networks on a chip to study the procoagulant effects of ambient fine particulate matter
Xu Yang(1), Yang Wu(2)
Added on: 07-09-2020
[1] https://pubs.rsc.org/en/content/articlelanding/2018/RA/C8RA90041H[2] https://www.aimbiotech.com/vasculogenesis.html
