Peptide assay for detection of sensitizing chemicals
Validated Method 2009
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
The DPRA supports the discrimination between sensitizing and non-sensitizing chemicals. It measures in chemico the binding of test chemicals to model synthetic peptides. In skin proteins, many amino acids contain electron-rich groups capable of reacting with sensitizers. Therefore, these peptides are used by the assay so they can react with electrophilic chemicals (skin sensitizers).
Validated and regulatory approved under TM2009-06 (EU) by EURL ECVAM (TSAR list), OECD Test No. 442C.
Direct Peptide Reactivity Assay
EURL ECVAM
Added on: 07-03-2020
[1] https://tsar.jrc.ec.europa.eu/test-method/tm2009-06[2] https://www.oecd-ilibrary.org/environment/test-no-442c-in-chemico-skin-sensitisation_9789264229709-en
