Skin sensitisation determination by CD86-marker
Validated Method 2013
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
The U-SENS test detects whether a chemical has a sensitizing or non-sensitizing effect on the skin.
This test is based on the flow cytometry measurement of a protein marker produced by U937 cells exposed to test chemicals. This reaction is one of the reactions of a key event of the Adverse Outcome Pathway (AOP), which is related to skin sensitization.
Validated and regulatory approved under TM2013-02 (EU) by EURL ECVAM (TSAR list), OECD Test No. 442E.
U-SENS™ - Myeloid U937 Skin Sensitisation Test
EURL ECVAM
Added on: 07-03-2020
[1] https://tsar.jrc.ec.europa.eu/test-method/tm2013-02[2] https://www.sciencedirect.com/science/article/abs/pii/S0887233315002489?via%3Dihub[3] https://www.oecd-ilibrary.org/environment/test-no-442e-in-vitro-skin-sensitisation_9789264264359-en
