Human organoids with autologous immune cells
2024
Institute of Human Biology (IHB), Basel, Switzerland
The epithelium-immune system relationship is crucial for maintaining tissue health, with disruptions linked to autoimmune diseases and cancer. While stem cell-derived organoids are valuable models for studying epithelial function, they lack immune cells required for organ-level processes. This study developed human intestinal immuno-organoids (IIOs) by combining epithelial organoids with autologous tissue-resident memory T (TRM) cells, a portion of which integrate into the epithelium and monitor the barrier. Using IIOs and single-cell transcriptomics, intestinal inflammation triggered by cancer-targeting biologics was studied, revealing activation of CD8+ T cells and a CD4+ T helper-1-like population. The Rho pathway was identified as a potential target to mitigate immunotherapy-associated intestinal inflammation, highlighting IIOs as a valuable tool for studying immune responses in cancer and autoimmune diseases.
Human organoids with an autologous tissue-resident immune compartment
Lauriane Cabon, Nikolche Gjorevski, Jarrett Gray Camp
Added on: 11-06-2024
[1] https://www.nature.com/articles/s41586-024-07791-5
