Fish cell acute toxicity test
Regulatory acceptedOECD Guidelines for the Testing of Chemicals, Paris, France
The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed using fluorescent cell viability indicator dyes measuring the metabolic activity, the integrity of the cell membrane, and the integrity of the lysosomal membrane.
The data are expressed as the percent cell viability of unexposed control values versus the test chemical concentration. The resulting concentration-response curves serve to determine the effective concentrations causing 50% loss in cell viability, i.e. the EC50 value.
RTgill-W1 cell line assay-derived EC50 values have been demonstrated to be in excellent agreement with lethal concentrations (LC50 values) determined in fish acute toxicity tests for a wide range of chemicals.
Test Guideline No. 249: Fish Cell Line Acute Toxicity
OECD
Added on: 04-15-2024
[1] https://www.oecd-ilibrary.org/environment/test-no-249-fish-cell-line-acute-toxicity-the-rtgill-w1-cell-line-assay_c66d5190-en
