Fish cell lines in determination of nanomaterial ecotoxicity
November 2018
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain
Graphene-related materials (GRMs) are commonly used and their release into aquatic environments is highly likely. The objective of this study was to investigate the usefulness of a battery of in vitro cytotoxicity assays applied on fish cells in order to generate ecotoxicity data for a number of GRMs including carbon nanofibres (CNFs) and graphene oxide (GO).
Two different fish cell lines were used: Topminnow fish hepatoma cell line (PLHC-1), due to the key role played by liver in detoxification, and a carp leukocyte cell line (CLC), due to the contribution of leukocytes to nanomaterial clearance. Cells were exposed to different concentrations of GRMs for 24 and 72 h and cell viability was assessed by measuring mitochondrial activity, plasma membrane integrity and lysosomal function. In both cell lines, CNFs appeared to have higher toxicity than GO. Intracellular fate of GRMs was investigated via transmission electron microscopy.
The results contribute to the understanding of the toxicity and behaviour of GRMs in living systems and could aid in designing safer materials for the environment.
Usefulness of fish cell lines for the initial characterization of toxicity and cellular fate of graphene-related materials (carbon nanofibers and graphene oxide)
Judit Kalman
Added on: 02-23-2024
[1] https://www.sciencedirect.com/science/article/pii/S0045653518322379?via%3Dihub
