Liver spheroids from rainbow trout
October 2018
University of Gothenburg, Gothenburg, Sweden
Piscine cell lines can be used to investigate the possible fate and effect of chemicals in fish. As reproduction of complex metabolic processes and toxic responses relies on organ-specific tissue architecture and functions, this study aims to develop three-dimensional (3D) spheroids of the continuous rainbow trout liver cell line RTL-W1.
RTL-W1 cells were used for spheroid production and different strategies were tested to prevent multi-spheroid cluster formation. Spheroid cultures were incubated with a test compound (β-naphthoflavone) for 24 hours. Analysis of cell viability and metabolic activity were performed and cytochrome P450 1A expression levels were analysed.
The results show that the RTL-W1 cells in 3D spheroids were viable, metabolically active and had higher β-naphthoflavone-induced cytochrome P450 1A expression levels than conventional 2D cell cultures. Furthermore, they displayed ultrastructural characteristics similar to differentiated hepatocytes. This suggests that the spheroids could be a superior model to assess hepatic biotransformation, bioaccumulation and chronic toxicity compared to conventional cell monolayer cultures.
Development of three-dimensional (3D) spheroid cultures of the continuous rainbow trout liver cell line RTL-W1
Tobias Lammel
Added on: 02-22-2024
[1] https://www.sciencedirect.com/science/article/abs/pii/S014765131831008X?via%3Dihub
