Self-organisation of spheroids by acoustic levitation
2024
École supérieure de physique et de chimie industrielles (ESPCI), Paris, France
Acoustic levitation (floating technique), which enables contactless manipulation of micro-objects with ultrasound, is a promising technique for the formation and culture of spheroids. This acoustofluidic technique favours cell-cell interactions away from the walls of the chip, leading to spontaneous self-organisation of the cells. Using this approach, spheroids were generated from mesenchymal stromal cells, liver and endothelial cells, and it was shown that long-term culture of cells in acoustic suspension is possible. It was also shown that this self-organisation and its dynamics are only weakly dependent on the acoustic parameters, but strongly dependent on the levitated cell type. Furthermore, spheroid organisation was altered by inhibitors of the actin cytoskeleton or by inhibitors of calcium-mediated interaction. These results confirm that acoustic levitation is an emerging technique for basic research and biotechnological industrial application in the rapidly growing field of microphysiological systems. It enabled the simple production of spheroids with specific and predictable shape and size that could be cultured for several days without the need for hydrogels or an extracellular matrix.
Acoustic levitation as a tool for cell-driven self-organization of human cell spheroids during long-term 3D culture
Lucile Rabiet, Jean-Luc Aider
Added on: 02-19-2024
[1] https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.28651
