Human-based assays for assessing the bioactivity and toxicity of environmental samples
CompanyINDIGO Biosciences, Inc., State College, USA
INDIGO Biosciences' human-based bioassay kits are designed for easily reproducible and comprehensive assessment of the bioactivity and toxicity of environmental samples. The standardized tests enable screening for known contaminants, such as estrogen, androgen and dioxin-like compounds, and provide meaningful results within 24 hours. For the analysis, the environmental samples are brought into contact with cultured reporter cells on a specially developed assay plate in which the bioluminescent enzyme luciferase has been integrated. The binding of the reporter cells to harmful compounds leads to a luminous reaction, which is determined quantitatively and serves as a sensitive surrogate measure for evaluating the functional activity of the respective receptor. Each kit contains luciferase reporter cells, an assay plate, all necessary reagents, and a technical manual to instruct you on the individual steps. A luminescence measuring device is also required for the RLU measurements (Relative Light Unit). To ensure homogeneity across batches, the company uses transiently transfected cells, which are less susceptible to genetic drift and fluctuations. In addition, they do not require prolonged cell cultivation, reducing the time required and reducing the risk of contamination. The cell kits are delivered frozen and are ready for use immediately after thawing. In summary, the all-inclusive luciferase reporter assay systems prove to be an effective method for assessing the toxic load of environmental samples (such as surface and wastewater, as well as recycled water) with disease-associated compounds. If required, INDIGO Biosciences carries out environmental testing as a service and also offers the development of customer-specific assays.
Environmental monitoring & testing assay kits
www.indigobiosciences.com
Added on: 02-13-2024
[1] https://indigobiosciences.com/environmental-testing-solutions/species-human/
