Rainbow trout cell lines for toxicity testing
November 2015
University of Waterloo, Waterloo, Canada
In this study two rainbow trout epithelial cell lines, the gill-derived cell line RTgill-W1 and the liver cell line RTL-W1, have been used to evaluate the toxic potential of six benzotriazoles (BTRs) and tolytriazole (TT). The cytotoxicity of BTRs was evaluated in 96-well plate cultures, where the cells were exposed to the BTRs for either 24 hours or 12 days. Oxidative stress was determined, the cell death mechanisms were investigated and genotoxicity of BTRs was tested using the comet assay.
Except for one, all other BTRs were cytotoxic at concentrations above 15 mg/L and transitorily elevated reactive oxygen species (ROS) levels, but ROS were not found to be the major cause of cell death. Genotoxicity was found for three BTRs. In summary, the toxic potential of BTRs appears to be limited to only high concentrations.
Use of the rainbow trout cell lines, RTgill-W1 and RTL-W1 to evaluate the toxic potential of benzotriazoles
Niels C. Bols
Added on: 02-06-2024
[1] https://www.sciencedirect.com/science/article/abs/pii/S0147651315301494?via%3Dihub
