Optimization of brain model cellular composition
2023
Bloomberg School of Public Health Johns Hopkins University, Baltimore, USA
Most brain organoid models are predominantly composed of different neuronal types, with a smaller percentage of glial cells present compared to in vivo conditions. The aim of this study was to expand the population of astrocytes and oligodendrocytes by using an optimized and chemically defined culture medium.
Brain models were differentiated from two induced pluripotent stem cell (iPSC) and two embryonic stem cell lines. It was found that by use of the optimized medium, astrocytes not only increased in number but also changed in morphology, more closely recapitulating primary culture astrocytes.
The optimized culture conditions have the potential to significantly improve microphysiological brain models for the study of neurological diseases and drug discovery.
A novel approach to increase glial cell populations in brain microphysiological systems
Lena Smirnova
Added on: 12-09-2023
[1] https://www.biorxiv.org/content/10.1101/2023.09.14.557775v1
