Heart and kidney axis-on a chip
2023
Leiden University Medical Center, Leiden, Netherlands
In this study, the design and development of a microfluidic unit to study the interaction of heart and kidney is described. This should allow investigating the interdependent relationship between these organs, as pathological changes in one of the organs can damage the other.
Cardiac microtissues (cMTs) and kidney organoids (kOs) were generated from human induced pluripotent stem cells (hiPSCs) and loaded into two separated but communicating chambers of a perfusion chip. Static culture conditions were compared with dynamic culture. Tissue viability was maintained for minimally 72 h, as indicated by the presence of beating structures in cMTs and the presence of nephron structures and albumin uptake in kOs.
The developed system enables the study of human cardiac and kidney organoid interaction and the investigation of diseases involving both organs and their potential treatments.
Heart and kidney organoids maintain organ-specific function in a microfluidic system
Milena Bellin
Added on: 12-09-2023
[1] https://www.sciencedirect.com/science/article/pii/S2590006423002788?via%3Dihub
