Modelling intestinal host-pathogen interactions
2022
The Barcelona Institute of Science and Technology, Barcelona, Spain
This study presents a human in-vitro model for intestinal host-pathogen interactions. The model resembles the small intestinal mucosa and includes the secreted mucus layer and a relevant 3D topography, as the epithelial barrier grows in a 3D villus-like hydrogel scaffold.
The 3D architecture significantly enhanced the secretion of mucus. Upon infection with a pathogenic E. coli strain, the mucus was shown to promote adhesion of bacteria while being protective for invasion of the bacteria. Accordingly, the degree of inflammatory response was milder when a thick mucus layer was present.
In summary, the model mimics the key features of the intestinal mucosa necessary to study the interactions with intestinal pathogens.
Mimicking the intestinal host-pathogen interactions in a 3D in vitro model: the role of the mucus layer
Elena Martínez, María García-Díaz
Added on: 09-08-2023
[1] https://www.mdpi.com/1999-4923/14/8/1552
