In vitro investigation of skin damage caused by air pollution
2017
Université de Pau et des Pays de l’Adour, Pau, France
Compelling evidence suggests that volatile organic compounds (VOCs) have potentially harmful effects to the skin. However, knowledge about cellular signalling events and toxicity after VOC exposure to human skin cells is still poorly documented. The aim of this study was to focus on the interaction between 5 different VOCs (hexane, toluene, acetaldehyde, formaldehyde and acetone) at doses mimicking chronic low level environmental exposure and the effect on human keratinocytes to get better insight into VOC-cell interactions. The researchers provide evidence that the proteasome, a major intracellular proteolytic system which is involved in a broad array of processes such as cell cycle, apoptosis, transcription, DNA repair, protein quality control and antigen presentation, is a VOC target. Proteasome inactivation after VOC exposure is accompanied by apoptosis, DNA damage and protein oxidation. Lon protease, which degrades oxidized, dysfunctional, and misfolded proteins in the mitochondria, is also a VOC target. Using human skin explants, it could be found that VOCs prevent cell proliferation and also inhibit proteasome activity in vivo. Taken together, these findings provide insight into potential mechanisms of VOC-induced proteasome inactivation and the cellular consequences of these events.
Oxidative damage and impairment of protein quality control systems in keratinocytes exposed to a volatile organic compounds cocktail
Anne-Laure Bulteau
Added on: 08-31-2023
[1] https://www.nature.com/articles/s41598-017-11088-1
