Single-molecule imaging to study molecules in live cells
2020
New York University School of Medicine, New York, USA(1)
Université de Montréal, Montreal, Canada(2)
Université de Montréal, Montreal, Canada(2)
Single-molecule imaging, an advanced microscopy technique, allows tracking the movements of tagged individual enzymes by immunofluorescence, thus enabling activity studies at a molecular level. The researchers showed that the telomerase continuously probes the telomeres. It was previously unclear how telomerase molecules are directed to the telomeres within the crowded nucleus. Mutations of a telomeric regulatory factor altered the activity, which contributes to the development of tumours. Thus, an important component of cancer development and ageing could be deciphered.
Single-molecule imaging of telomerase RNA reveals a recruitment-retention model for telomere elongation
Agnel Sfeir(1), Pascal Chartrand(2)
Added on: 06-24-2020
[1] https://www.sciencedirect.com/science/article/abs/pii/S1097276520303063?via%3Dihub[2] https://www.bionity.com/en/news/1166587/tracking-cancer-s-immortality-factor.html?pk_campaign=ca0264&WT.mc_id=ca0264
