Human CNS barrier-forming organoids with cerebrospinal fluid production
2020
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
The cerebrospinal fluid (CSF) is a vital fluid that provides nutrients and signalling molecules and removes toxic by-products from the brain. CSF is produced by the choroid plexus (ChP), a protective epithelial barrier that also prevents the free entry of blood. Here, human ChP organoids were established with a selective barrier and CSF-like fluid secretion in self-contained compartments. This in vitro barrier has been shown to have the same small molecule selectivity as in vivo, and ChP-CSF organoids can predict CNS permeability of novel compounds. The transcriptomic and proteomic signature of ChP-CSF organoids showed a high degree of similarity to in vivo. Finally, overlap of single-cell transcriptomics and proteomic analysis revealed important human CSF components produced by previously unidentified specialized epithelial subtypes.
Human CNS barrier-forming organoids with cerebrospinal fluid production
Madeline A. Lancaster
Added on: 05-11-2023
[1] https://www.science.org/doi/10.1126/science.aaz5626
