Cultivation of 3D brain organoids without Matrigel
2021
UConn School of Medicine, Farmington, USA
Here, the generation of 3D organoid cultures is described, which are generated with a technique called Neurosphere-derived organoid-like aggregates by orbital shaking (NEDAS). This protocol takes advantage of the fusion behavior of neurospheres by generating three-dimensional aggregates without the need for Matrigel like the typical cerebral organoids. NEDAS exhibit proliferation, differentiation and apoptosis with characteristic self-organization. Furthermore, NEDAS have areas with corridors of Neural Progenitor Cells (NPCs) that express markers and features of migratory cells. In addition, the expression of GABAergic and excitatory neurons is shown.
Generation of neurosphere-derived organoid-like-aggregates (NEDAS) from neural stem cells
Jaime Imitola
Added on: 04-18-2023
[1] https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.15
