In-vitro skin models of wound healing
2023
Association of Dutch Burn Centres, Beverwijk, Netherlands(1)
Vrije Universiteit Amsterdam, Amsterdam, Netherlands(2)
Vrije Universiteit Amsterdam, Amsterdam, Netherlands(2)
Healing of burn injury is a complex process that often leads to the development of functional and aesthetic complications. To study skin regeneration in more detail, full skin equivalents generated on the basis of human de-epidermalized dermis and different collagen matrices were developed and investigated.
Proper dermal and epidermal morphogenesis was established by coculturing human keratinocytes and fibroblasts derived from skin tissue samples originating from surgeries involving removal of excess skin. After applying a standardized burn injury to the models, reepithelization was observed 2 weeks after injury. High levels of pro-inflammatory cytokines were present in the culture media of all models.
It is anticipated that the in-vitro models can facilitate research on skin regeneration and can be used to test therapeutic interventions in a preclinical setting to improve wound healing.
Full skin equivalent models for simulation of burn wound healing, exploring skin regeneration and cytokine response
Bouke K. H. L. Boekema(1), Patrick P. G. Mulder(1), Esther Middelkoop(2)
Added on: 04-03-2023
[1] https://www.mdpi.com/2079-4983/14/1/29
