A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils
2012
Korea University, School of Mechanical Engineering, Seoul, South Korea
Leukocyte transendothelial migration (TEM) is a multi-step process in the inflammatory response that plays critical roles in the defence against invading pathogens (triggered by bacteria and its degradation products entering the body by wounds) and the development of diverse immune disorders, as well as abnormal collagen stiffness, including angiogenesis and cancer-cell metastasis.
This method allows for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transendothelial migration of neutrophils during the inflammatory process, induced by various inflammatory stimuli. It can enable the anaylsis of the interplay among various immune cells, different types of endothelial cells and other blood cells. This device can be used for phenotype-based, high-throughput drug screening.
A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils
Seok Chung
Added on: 05-29-2020
[1] https://pubs.rsc.org/en/content/articlelanding/2012/LC/c2lc40445a#!divAbstract
