iGIST - a kinetic bioassay for pertussis toxin
November 2020
University of Turku, Turku, Finland
Detection of pertussis toxin (PTX) activity is instrumental in the development and manufacturing of pertussis vaccines. Here, the researchers describe Interference in Gαi-mediated Signal Transduction (iGIST), an animal-free kinetic bioassay for the detection of PTX, by measuring its effect on inhibitory G protein-coupled receptor (GPCR) signalling. iGIST is based on HEK293 cells and a luminescent 3'5'-cyclic adenosine monophosphate (cAMP) probe. iGIST has a low sensitivity threshold in the pg/mL range of PTX, surpassing by 100-fold in a parallel analysis via the currently used in vitro end-point technique to detect PTX. iGIST also detects PTX in complex samples, i.e., a commercial PTX-toxoid-containing pertussis vaccine that was spiked with an active PTX. iGIST has an objective digital readout and is observer-independent, offering prospects for automation. iGIST emerges as a promising method to detect PTX activity in the development and manufacturing of pertussis vaccines.
iGIST— a kinetic bioassay for pertussis toxin based on its effect on inhibitory GPCR signaling
Arto T. Pulliainen
Added on: 10-26-2022
[1] https://pubs.acs.org/doi/10.1021/acssensors.0c01340
