Small intestine-on-a-chip using biopsy-derived organoids show physiological functions and near in-vivo- gene expression
2018
Harvard University, Boston, USA
The intestinal chip contains human intestinal epithelial cells derived from organoids or biopsies as well as tissue-specific microvascular endothelial cells of the intestine. Among other things, it exhibits 3D intestinal villi-like structures, multi-line differentiation, epithelial barrier function, enzymatic activity of the brush border enzyme and mucus production. Together with intestinal stem cells there are nutrient-digesting and absorbing enterocytes, mucus-producing goblet cells, hormone-secreting enteroendocrine cells, and microbiome-regulating and sensory Paneth cells. Peristalsis and nutrient flow are also simulated. The transcriptome of the intestinal chip is similar to that of the adult human duodenum in vivo. Since the microenvironment can be maintained for weeks, they allow both short and longer-term studies; in the long term, this will allow a better understanding of human-microbiome interactions, the modelling of malnutritional and inflammatory bowel disorders, and the performance of personalised drug testing.
Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids
Donald E. Ingber
Added on: 05-26-2020
[1] https://www.nature.com/articles/s41598-018-21201-7[2] https://www.technologynetworks.com/cell-science/news/from-human-biopsy-to-complex-gut-physiology-on-a-chip-297510
