In-vitro model of peripheral neuropathies
2022
University of Konstanz, Konstanz, Germany
In vitro models of the human peripheral nervous system (PNS) are required to study chemotherapy-induced peripheral neuropathy (CIPN) and other impairments of the PNS. In this study, induced pluripotent stem cells (iPSCs) were harnessed to generate peripheral sensory neurons enriched in nociceptors and a corresponding protocol was established.
After extensive phenotypic profiling, Ca2+-imaging was chosen as a quantitative endpoint for the assessment of pain-receptor signalling. A case study with the chemotherapeutic drug oxaliplatin at non-cytotoxic concentrations was performed to demonstrate the relevance of the novel PNS model. Neuronal hypersensitivity to otherwise non-activating mechanical stimulation was found that could be blocked by modulators of voltage-gated sodium channels.
These findings indicate that the model is suitable for pharmacological and toxicological studies related to peripheral neuropathies.
Generation of human nociceptor-enriched sensory neurons for the study of pain-related dysfunctions
Marcel Leist
Added on: 09-01-2022
[1] https://academic.oup.com/stcltm/article/11/7/727/6605938
