Hyaluronan-laminin hydrogels for 3D neuronal cell culture
2022
KTH Royal Institute of Technology, Stockholm, Sweden(1)
Linköping University, Linkoping, Sweden(2)
Linköping University, Linkoping, Sweden(2)
In this work, a 3D bioprinting-compatible modular hyaluonan-based hydrogel system was developed that allows for convenient integration and efficient retention of recombinant laminin, which enhances the survival, proliferation, and differentiation of neuroepithelial stem cells. Laminin presenting hydrogels were cross-linked by copper-free click chemistry, thereby enabling the tuning of material properties and creation of biologically relevant microenvironments.
The resulting hydrogels supported encapsulation and culture of both, human neuroblastoma cells (SH-SY5Y) and human induced pluripotent stem cell (hiPSC)-derived neuroepithelial stem cells.
The tunable rheological properties of the hydrogels provided a protective effect on the neuroepithelial stem cells during syringe extrusion in an in vitro model for cell injection therapy. In addition, 3D bioprinting of the cell-laden hydrogels allowed for the fabrication of structurally well-defined constructs with high cell viabilities, facilitating the development of tissue and disease models.
Bioorthogonally cross-linked hyaluronan-laminin hydrogels for 3D neuronal cell culture and biofabrication
Daniel Aili(1, 2), Anna Herland(1)
Added on: 08-31-2022
[1] https://onlinelibrary.wiley.com/doi/10.1002/adhm.202102097
