Investigating of an oncolytic virotherapy using breast cancer organoids
2022
University of Tuebingen, Tuebingen, Germany
An established three-dimensional organoid model derived from the tissue of 10 patients with primary breast cancer was used to develop an experimental protocol for infecting organoid cultures with oncolytic viruses. The oncolytic effects of a measles vaccine virus and a vaccinia virus genetically engineered to express either fluorescent proteins or a suicide gene were investigated. The method demonstrated that all oncolytic viruses significantly inhibited cell viability in organoid cultures derived from breast cancer tissue. The model provides a promising in-vitro method to help further testing and engineering of new generations of virotherapeutic vectors.
A three-dimensional organoid model of primary breast cancer to investigate the effects of oncolytic virotherapy
André Koch
Added on: 04-22-2022
[1] https://www.frontiersin.org/articles/10.3389/fmolb.2022.826302/full
