Bioprinted neuroblastoma model for drug testing
December 2021
Technische Universität Berlin, Berlin, Germany
A three-dimensional model for neuroblastoma composed of a human neuroblastoma cell line (IMR-32) in a renal environment made of human embryonic kidney cells and primary human kidney fibroblasts is demonstrated. The model was produced using a commercially available bioprinter. In this simplified metastasis model, neuroblastoma cells were surrounded by a microenvironment made up of human kidney cells. Two drugs were exemplarily tested in this model: While one of them selectively killed the cancer cells by apoptosis induction but did not affect the renal cells in the therapeutically effective concentration range, the other induced cell death in both cell types. This cancer model allows the testing of cytotoxicity and tumour selectivity of new anticancer drugs, and the open scaffold design enables the free exchange of tumour and microenvironment by any cell type.
Bioprinted cancer model of neuroblastoma in a renal microenvironment as an efficiently applicable drug testing platform
Jens Kurreck
Added on: 04-22-2022
[1] https://www.mdpi.com/1422-0067/23/1/122
