Lung cancer organoids from pleural effusion aspirate
2019
Wake Forest School of Medicine, Winston-Salem, USA
In this study, the use of 3D hydrogel-based lung cancer organoids derived from pleural effusion aspirate from multiple lung cancer patients is demonstrated. The resulting organoids preserve the phenotype of the cancer cells as well as maintain cancer and stromal cell interactions. Patient-derived cells placed directly into hydrogel-based organoids created anatomically relevant structures and exhibited lung-cancer-specific behaviour. On the other hand, cells first grown in plastic dishes and then cultured in 3D did not create similar structures. Moreover, chemotherapeutic responses of patient-derived cells were compared for 2D and 3D cell culture systems. Cells in 2D culture were more sensitive to treatment when compared with 3D organoids. Organoids from pleural effusion fluid of lung cancer patients display in-vivo-like anatomy and drug response and thus could serve as more accurate disease models for the study of tumour progression and drug development.
Pleural effusion aspirate for use in 3D lung cancer modeling and chemotherapy screening
Shay Soker
Added on: 03-25-2022
[1] https://pubs.acs.org/doi/10.1021/acsbiomaterials.8b01356
