Neurodevelopmental in vitro toxicity assessment
2021
Heinrich-Heine-University, Duesseldorf, Germany
In this study, human-induced pluripotent stem cell (hiPSC)-derived neural crest cells (NCC), mesencephalic cells (LUHMES), 3D human primary neural progenitor cell (NPC)-based neurospheres, as well as hiPSC-derived peripheral neurons were applied to study distinct neurodevelopmental key events. These key events include proliferation, migration, and differentiation of tested cells as well as investigation of cell morphology. The assay battery was tested by evaluating the neurodevelopmental toxicity of several fume retardants. Moreover, RNA sequencing was performed.
This approach represents a case study for a new risk assessment paradigm for neurodevelopmental toxicity by using phenotypic readouts of human cell-based assays that cover a variety of neurodevelopmental key events and studying their molecular signatures in response to different fume retardants. Using a human cell-based neurodevelopmental toxicity assessment (DNT) in vitro battery for hazard assessment is a promising approach for future risk assessment procedures.
Neurodevelopmental toxicity assessment of flame retardants using a human DNT in vitro testing battery
Ellen Fritsche
Added on: 03-17-2022
[1] https://link.springer.com/article/10.1007/s10565-021-09603-2
