HIV infection: Better understanding of the reservoir of virus in the body
December 2021
LMU München, Munich, Germany
CD4+ T cells are central mediators of adaptive and innate immune responses and constitute a major reservoir for human immunodeficiency virus (HIV) in vivo. Detailed investigations of resting human CD4+ T cells have been precluded by the absence of efficient approaches for genetic manipulation limiting our understanding of HIV replication and restricting efforts to find a cure. Here the researchers report a method for rapid, efficient, activation-neutral gene editing of resting, polyclonal human CD4+ T cells. Several genetic knockouts and knock-ins were generated, enabling the study of the physiological interplay of cellular and viral components at single-cell resolution. Together, this technique allows improved molecular and functional characterizations of HIV biology and general immune functions in resting CD4+ T cells.
Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4+ T cells allows complex functional analyses
Manuel Albanese, Oliver T. Keppler
Added on: 03-16-2022
[1] https://www.nature.com/articles/s41592-021-01328-8[2] https://www.bionity.com/en/news/1174173/hiv-infection-better-understanding-the-reservoir-of-virus-in-the-body.html?utm_source=newsletter&utm_medium=email&utm_campaign=bionityde&WT.mc_id=ca0264
