High-throughput transcriptomics platform for screening environmental chemicals
2021
U.S. Environmental Protection Agency, Durham, USA
To accelerate the pace of chemical risk assessments, there is a crucial need to develop new approach methodologies (NAMs) that efficiently provide information about chemical hazards. In the present study, the researchers aimed at leveraging the power of a new whole-transcriptome sequencing assay called TempO-Seq and using it for comprehensive and efficient bioactivity screening of environmental chemicals. The researchers used human cell line MCF7 as the in vitro biological model. The experimental design integrated a small collection of known chemicals to develop robust laboratory and bioinformatics workflows for generating high-throughput transcriptomics data by TempO-Seq in concentration-response that could be scaled up towards testing of 1000s of chemicals in parallel. All bioinformatics workflows were generated using open-source tools to facilitate uptake of the technology. The newly generated assay was validated and compared with existing methods. In conclusion, the study has resulted in a novel and scalable in vitro transcriptomics workflow that is suitable for high-throughput hazard evaluation of environmental chemicals.
High-throughput transcriptomics platform for screening environmental chemicals
Joshua A Harrill
Added on: 12-22-2021
[1] https://academic.oup.com/toxsci/article-abstract/181/1/68/6128532
