3D model to investigate metabolic response of lung cancer cells
Company 2016
Harvard University, Cambridge, USA(1)
Vertex Pharmaceuticals Incorporated, Boston, USA(2)
Vertex Pharmaceuticals Incorporated, Boston, USA(2)
In previous studies, a paper-based 3D culture system was developed, which can generate oxygen and nutrient gradient along the different layers. Here, it was used to investigate metabolic responses of human lung adenocarcinoma cells to ionizing radiation by calculating the availability of nutrients and oxygen in each layer, depending on the distance from the source, correlated to the metabolic activity of the cells. The results showed that there was a positive correlation between distance from the source and increased markers of hypoxia and metabolic stress and reduced sensitivity to ionizing radiation, replicating features of in vivo cancer cell behaviour in solid tumors. Finally, three different isogenic variants were identified, corresponding to the different types of metastatic behaviour in vivo. Overall, the researchers demonstrate that this 3D paper-based model can reproduce some characteristics of radiosensitivity of cancer cells to perform radiation response studies without the influence of cell migration and proliferation and controlling metabolic parameters at different time-points.
Metabolic response of lung cancer cells to radiation in a paper-based 3D cell culture system
George M Whitesides(1), Brenda K Eustace(2)
Added on: 11-28-2021
[1] https://www.sciencedirect.com/science/article/abs/pii/S0142961216300114[2] https://data.jrc.ec.europa.eu/dataset/176d71e6-5082-4b29-8472-b719f6bda323
