Microfluidic 3D model of lung cancer and tumour microenvironment
2016
Dalian Medical University, Dalian, China(1)
The People’s Hospital of Peking University, Beijing, China(2)
The People’s Hospital of Peking University, Beijing, China(2)
The tumour microenvironment is a key element in cancer development comprised of cancer and stromal cells, like cancer-associated fibroblasts, which have a central role in tumour progression. Additionally, another component that has been shown to be implicated in cancer progression is glucose-regulated protein 78. Here, a microfluidic 3D co-culture device is developed with human lung adenocarcinoma cells and fibroblasts to mimic tumour microenvironment and investigate the underlying mechanisms of tumour invasion. The results showed that cancer-associated fibroblasts induced cell migration and glucose-regulated protein 78 expression in lung cancer cells. Further confirmation of glucose-related protein 78 involvement in cell invasion was obtained through knockdown strategies, which blocked cancer cell metastatic behaviour. Overall, the researchers elucidate a major role of glucose-regulated protein 78 in cancer cell migration and propose a new microfluidic 3D device that can be used to investigate the interactions of cancer and stromal cells with the tumour microenvironment.
Cancer-associated fibroblasts promote non-small cell lung cancer cell invasion by upregulation of glucose-regulated protein 78 (GRP78) expression in an integrated bionic microfluidic device
Qi Wang(1), Zhancheng Gao(2)
Added on: 11-26-2021
[1] https://www.oncotarget.com/article/8232/[2] https://data.jrc.ec.europa.eu/dataset/176d71e6-5082-4b29-8472-b719f6bda323
