A tumor organoid model for studying breast cancer
2021
The Second Hospital of Dalian Medical University, Dalian, China
Malignant pleural effusion (MPE) is a serious medical condition in patients with advanced breast cancer (BC). Here, organoid culture technology was applied to culture preoperative puncture specimens and corresponding tumour cells derived from surgical specimens from early-stage BC patients and tumour cells from pleural effusion from an advanced triple-negative BC patient (TNBC) with MPE to investigate whether in vitro models can predict therapies for clinical patients. The organoids matched the histological features of primary BC and showed negative expression of TNBC biomarkers. A 3D drug screening test in combination with the clinical medication situation of the respective patient was performed. The pleural effusion-derived tumour organoids were sensitive to capecitabine and everolimus as single agent treatments. Sensitivity to capecitabine was consistent with this patient's clinical response to capecitabine and with sequencing results. The study concludes that an effective platform for ex vivo pleural effusion tumour organoids from advanced TNBC patients with MPE could be used to identify treatment options and investigate the clinicopathological characteristics of these patients.
Breast cancer organoids from malignant pleural effusion-derived tumor cells as an individualized medicine platform
Zuowei Zhao
Bo Pan et a. In Vitro Cellular & Developmental Biology - Animal 2021 [1]
Physicians Committee for Responsible Medicine [2]
Added on: 11-05-2021
[1] https://link.springer.com/article/10.1007%2Fs11626-021-00563-9[2] https://www.pcrm.org/news/ethical-science/nonanimal-research-model-studying-breast-cancer#:~:text=Study%20in%20a%20Sentence%3A%20Using,to%20test%20personalized%20treatment%20options.
