Development of a microfluidic device for drug screening for Raman measurements
2018
Utah State University, Logan, USA
In recent years, Raman spectroscopy has gained importance in cell classification. However, it is still difficult to apply it to cells cultured in microfluidic devices based on PDMS due to the background noise generated by this material. Here, a novel microfluidic device is used to culture human lung and breast cancer cells to perform Raman spectroscopy and study their response to doxorubicin. The results showed that the new device was biocompatible with the cancer cell lines and that their biomechanical properties were not altered. Furthermore, doxorubicin induced significant changes between treated and untreated cells in the Raman spectroscopy profiles, as reflected by the clustering in principal component analysis. Overall, the researchers develop a new microfluidic device that can be used for in vitro Raman measurements of living cells to investigate drug response and fast drug screening.
Microfluidic chip for non-invasive analysis of tumor cells interaction with anti-cancer drug doxorubicin by AFM and Raman spectroscopy
Anhong Zhou
Added on: 11-02-2021
[1] https://aip.scitation.org/doi/10.1063/1.5024359[2] https://data.jrc.ec.europa.eu/dataset/176d71e6-5082-4b29-8472-b719f6bda323
