3D platform to perform drug screening for lung cancer
2016
Dalian Medical University, Dalian, China
In vitro cell models are a popular choice to investigate the mechanisms of cancer development and perform drug testing. However, there are still severe limitations to replicating accurately the biological processes that drive non-small-cell lung cancer. Here, a hydrogel-based 3D culture system is used to reproduce an appropriate tumor microenvironment to culture human lung cancer cells and improve drug testing. The results showed good viability of cancer cells that formed aggregates with relevant tissue-like morphology. Moreover, EGF and EGFR were upregulated in 3D cultures, which enabled the researchers to reliably corroborate that EGFR inhibitors could inhibit aggregate growth and induce drug resistance. Overall, the researchers develop a new 3D platform that improves the in vitro modelling of lung cancer and provides a solid tool for drug discovery and testing.
Bioengineering three-dimensional culture model of human lung cancer cells: an improved tool for screening EGFR targeted inhibitors
Xiu-Li Wang, Li-Jun Yu
Added on: 11-02-2021
[1] https://pubs.rsc.org/en/content/articlelanding/2016/RA/C6RA00229C[2] https://data.jrc.ec.europa.eu/dataset/176d71e6-5082-4b29-8472-b719f6bda323
